Uvod
The combination of improved CTAB extraction method and column purification technology is suitable for extracting high-purity RNA from various plant/fungal samples, posebno biljna tkiva bogata polisaharidima i polifenolima (such as cotton leaves, mature rice leaves, Arabidopsis seeds, white pine needles, loquat leaves, roses, itd.). The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+purification, zaštita nukleinske kiseline, i in vitro prijevod. This product does not contain gvanidin isothiocyanate throughout the entire process, and the purified RNA product does not contain guanidine isothiocyanate contamination, making A260/230 more stable.
pojedinosti
Sadržaj kompleta
Sadržaj | R4156 |
Vremena pročišćavanja | 100 Pripreme |
Column B10 | 2 x 100 |
2ml cijevi za prikupljanje | 2 x 100 |
Međuspremnik PAL | 100 ml |
Buffer PRC1 | 60 ml |
Buffer SW1 | 60 ml |
Buffer SW2 | 110 ml |
Voda bez RNaze | 20 ml |
Skladištenje i stabilnost
The kit components can be stored at room temperature (15-25°C) i stabilni su najmanje 18 mjeseca.
Recenzije
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