HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sekvenciranje, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Recover DNA fragments between 60bp-10kbp from agarose gel |
| Prijave | PCR, NGS, labeling, ligation and enzyme digestion, itd. |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Agarose gel, PCR products, enzyme products |
| Količina uzorka | Agarose gel: ≤500mg |
| Oporavak | ≥80% |
| Volumen eluacije | ≥15μl |
| ≤20 minutes（1-24 samples） | |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 35µg |
Načelo
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Prednosti
- High recovery efficiency – ≥80% DNA recovery
- Općenito – recover DNA from gel or enzyme-driven reaction solutions such as PCR
- Fast – isolation can be completed in 10-15 minutes by column gel method
Sadržaj kompleta
| Sadržaj | D211102 | D211103 |
| Vremena pročišćavanja | 100 Pripreme | 250 Pripreme |
| Buffer GDP | 120 ml | 250 ml |
| Buffer DW2 | 50 ml | 2 x 50 ml |
| Elucijski pufer | 20 ml | 30 ml |
| HiPure DNA mini kolone II | 100 | 250 |
| 2 ml cijevi za prikupljanje | 100 | 250 |
Skladištenje i stabilnost
The Kit should be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Ako se u puferima stvori bilo kakav talog, zagrijte na 37 ℃ da se otopi.
Podaci o eksperimentu
Recenzije
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