The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA(mRNA) pročišćavanje, nuclease protection and in vitro translation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| Prijave | RT-PCR, qPCR, Sjeverna hibridizacija, Poly A purification, nucleic acid protection and in vitro translation translation |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, Tehnologija filtracije DNA |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Simple plant, petal, peel, seed |
| Količina uzorka | 50-150mg |
| Volumen eluacije | ≥300μl |
| Vrijeme po vožnji | ≤15 minutes（1-24 samples） |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Prednosti
- Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
- Visoka kvaliteta – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Brzo – može se izdvojiti nekoliko uzoraka 15 minutes by column method
- Wide range – samples including animals, biljke, bacteria, stanice, itd
- High output – large sample amount, and the yield can be up to 200μg
Sadržaj kompleta
| Sadržaj | R401402 | D401403 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 250 |
| PlantZol Reagent | 60 ml | 200 ml |
| Buffer GXP | 30 ml | 150 ml |
| Međuspremnik RW1 | 50 ml | 200 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Recenzije
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