Uvod
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
| Prijave | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, itd. |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | FFPE slice, FFPE embedded tissue |
| Količina uzorka | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
Načelo
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, dok se protein ne adsorbira i uklanja se filtracijom. Nakon pranja proteina i drugih nečistoća, RNA was finally eluted with low-salt buffer. Za DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, dok se protein ne adsorbira i uklanja se filtracijom. Nakon pranja proteina i drugih nečistoća, DNA was finally eluted with low-salt buffer.
Prednosti
- Visoka kvaliteta – high purity total RNA / DNA can be directly used in various downstream applications
- Brzo – column method can complete the extraction of several samples in 30 minuta
- Sef – no phenol chloroform extraction required
- Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Sadržaj kompleta
| Sadržaj | IVD5116 |
| Vremena pročišćavanja | 50 Pripreme |
| HiPure DNA Micro Column | 50 |
| HiPure RNA Mini Column I | 50 |
| 2ml cijevi za prikupljanje | 150 |
| Proteinaza K | 50 mg |
| Pufer za otapanje proteaze | 5 ml |
| Buffer DPS | 60 ml |
| Buffer FRL | 15 ml |
| Buffer ATL | 15 ml |
| Buffer RLC | 15 ml |
| Buffer AL | 15 ml |
| Buffer VHB | 44 ml |
| Buffer RW2 | 25 ml |
| Voda bez RNaze | 10 ml |
| Međuspremnik AE | 10 ml |
Skladištenje i stabilnost
Proteinazu K treba čuvati na 2-8°C po dolasku. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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