Uvod
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin stupac to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Co-isolation DNA and RNA from a single sample (stanice, soft tissue, plant sample) |
| Prijave | Rt-PCR, cDNA synthesis, PCR andsecond-generation sequencing, itd. |
| Metoda pročišćavanja | Mini spin kolona |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Količina uzorka | Soft Tissue: < 30mg, Cell: 1 x 107, Plant: 50 – 100mg |
| Prinos | DNA: 2 – 20 μg, RNA: 5 – 50 μg |
Načelo
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Prednosti
- Visoka kvaliteta – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Brzo – column method can complete the extraction of several samples in 30 minuta
- Sef – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Sadržaj kompleta
| Sadržaj | R511102 | R511103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Međuspremnik DW1 | 30 ml | 150 ml |
| Međuspremnik RW1 | 30 ml | 150 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
| Međuspremnik AE | 10 ml | 50 ml |
Skladištenje i stabilnost
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
Još nema recenzija.