HiPure DNA Nano Column

Uvod

• HiPure Columns by Magen Biotech:
  • Prepared using high-quality glass fiber filter membrane.
  • Production processes include membrane cutting, release, ring pressing, gland, and weighing.
  • Characteristics: long-term stability and high binding capacity.
  • Experiments demonstrate unchanged binding capacity and efficiency when stored at room temperature for 4 godina.
• Nucleic Acid Columns Series:
  • Based on imported glass fiber membranes (GF/B, GF/D, GF/F).
  • Production processes are strictly controlled, including material selection, injection molding, and membrane packing.
  • Ensures extremely high adsorption capacity and long-term stability.
  • Varieties available, with binding rate unchanged when stored at room temperature for 4 godina.

Tehnički podaci

Adsorption Mechanism

DNA/RNA Binding Process:

1. Affinity to Glass Fibers:
   • Based on the negatively charged DNA skeleton, it exhibits a high affinity for positively charged glass fibers.
2. Interaction with Silica Gel:
   • In high salt and ethanol solutions, DNA/RNA binds to glass fibers and interacts with the hydrophilic matrix on silica through hydrogen bonds.
   • This interaction results in tight binding of DNA/RNA to the silica gel membrane.
3. Pollutant Removal:
   • Washing the solution removes all pollutants.
   • At high salt concentrations, nucleic acids selectively bind to the silica gel membrane, while other pollutants, primarily proteins, are removed by membrane washing.