This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plazma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija i cirkulirajuća DNA/RNA (include miRNA) iz 1-5 ML serum i plazma |
| Prijave | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| Metoda pročišćavanja | Midi spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, Tehnologija filtracije DNA |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | serum, plazma, and other cell-free liquid samples |
| Količina uzorka | 1-5 ml |
| Volumen eluacije | ≥20μl |
| Vrijeme po vožnji | ≤100 minutes |
| Nosivi volumen tekućine po stupcu | 4 ml |
| Prinos vezivanja kolone | 1 mg |
Načelo
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Prednosti
- Visok prinos – most optimized process to obtain maximum free RNA and small RNA
- High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
- Visoka čistoća – low alcohol combination, completely remove inhibitor and protein pollution
- Visoki oporavak – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
Sadržaj kompleta
| Sadržaj | R431602 | D431603 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Micro Columns | 50 | 5 x 50 |
| HiPure Viral Midi Columns | 50 | 5 x 50 |
| 15 ml cijevi za prikupljanje | 50 | 5 x 50 |
| 2ml cijevi za prikupljanje | 50 | 5 x 50 |
| Buffer CFL | 150 ml | 2 x 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 x 250 ml |
| Međuspremnik RW2* | 2 x 50 ml | 5 x 100 ml |
| Voda bez RNaze | 10 ml | 50 ml |
Skladištenje i stabilnost
The kit components can be stored at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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