HiPure Circulating DNA Midi Spin Kit D (Spin protokol)

Uvod

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:

• Targets:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Types:
  • Human plasma
  • Human serum
  • Human urine (fresh or frozen once)
• Focus on Short DNA: Captures short nucleic acids (<1000bps) commonly found in biofluids.
• Efficient Purification: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.

Benefits:

• Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• Broad Sample Compatibility: Works with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.

pojedinosti

Tehnički podaci

Značajke	Tehnički podaci
Glavne funkcije	Isolation circulating DNA from 1-5ml plasma, serum, body fluids by spin
Prijave	qPCR, liquid or solid chip analysis, hybridization, and SNP detection, itd.
Metoda pročišćavanja	Mini spin stupac
Tehnologija pročišćavanja	Silika tehnologija
Procesna metoda	Priručnik (centrifugation)
Vrsta uzorka	Serum, plazma, and other cell-free fluid samples
Količina uzorka	1-5ml
Volumen eluacije	≥50μl
Vrijeme po vožnji	≤70 minuta
Nosivi volumen tekućine po stupcu	4ml
Prinos vezivanja kolone	1mg

Načelo

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. Washing: Wash buffers to remove leftover proteins and cellular debris.
4. Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Prednosti

• Visok prinos – most optimal process, free DNA (>50bps) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• Visoka čistoća – low alcohol binding method, completely removing inhibitor and protein pollution
• Visoki oporavak – DNA can be recovered at the level of PG by silica gel column purification

Sadržaj kompleta

Sadržaj	D318203D
Vremena pročišćavanja	50 Pripreme
Buffer ACL	250 ml
Buffer ACB*	300 ml
Buffer DCW1*	22 ml
Buffer DCW2*	10 ml
Proteinaza K	540 mg
Pufer za otapanje proteaze	30 ml
Carrier RNA	110 μg
Nuclease Free Water	20 ml
HiPure CFDNA Mini Columns	50
2 ml cijevi za prikupljanje	100
Extender Tubes	50
Sealing O-Ringes	50
50ml Collection Tube	50
Support Tube	50

Skladištenje i stabilnost

• Proteinaza K & Carrier RNA: Fridge (2-8°C) for best results.
• Everything Else: Room temperature (15-25°C) is fine.
• Important: If storing cold, warm buffers before use.