The Kit provides fast purification of high-quality RNA from whole blood, stanice, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA (mRNA) pročišćavanje, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija ukupne RNA od 1-1,5 ml cjelovite krvi |
| Prijave | qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, acid phenol/gvanidin extraction technology (MagZol pretreatment technology) |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Fresh or frozen blood, mammalian blood |
| Količina uzorka | ≤1.5 ml whole blood |
| Prinos | 2-100μg |
| Volumen eluacije | ≥20μl |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100μg |
Načelo
The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.
Prednosti
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Brzo – izolacija nekoliko uzoraka može se dovršiti u 20 minuta pomoću metode pročišćavanja kolonom
- Osjetljiv – RNA can be purified at the level of PG
Sadržaj kompleta
| Sadržaj | R416102 | R416103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA mini stupci I | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 500 |
| 10 x Buffer RBC | 50 ml | 3 x 100 ml |
| RTL Lysis Buffer | 50 ml | 250 ml |
| Međuspremnik RW1 | 50 ml | 250 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.
Recenzije
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