Bilirubine directe Solarbio (DBIL) Kit d'analyse 50T | 48S

Note: Prélevez deux ou trois échantillons différents pour la prédiction avant le test.

Équipement d'exploitation: Spectrophotomètre/Lecteur de microplaques

Numéro de catalogue: BC5175

Taille: 50T | 48S

Composants:

Réactif I: 25 mL×1. Conserver à 2-8 ℃.

Réactif II: 6 mL×1. Conserver à 2-8 ℃.

Description du produit：

Bilirubine directe (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 nm. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 nm.

Réactifs et équipements requis mais non fournis：

Spectrophotomètre/Lecteur de microplaques, centrifugeuse de bureau, constant temperature foster box/water bath, pipette, cuvette en verre micro/plaque à fond plat de 96 puits, glace, et eau distillée.

Procédure

UN. La préparation des échantillons:

Sérum, plasma, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

B.Détermination procédure：

1. Preheat the spectrophotometer/microplate reader for 30min, ajuster la longueur d'onde à 450 nm, and set the spectrophotometer counter to zero with distilled
2. Détermination:

Réactif (µL)	Tube à essai	Tube vierge
Échantillon	8	–
Eau distillée	–	8
Réactif I	192	192
Bien mélanger. React at 37℃ for 5 minutes. Mesurer l'absorbance à 450 nm, and record it as A1T, A1b;
Réactif II	48	48
Bien mélanger. React at 37℃ for 5 minutes. Mesurer l'absorbance à 450 nm, and record it as A2T, A2B. Calculate the ΔAT =A1T-A2T and the ΔAB =A1B-A2B. Blank tubes only need to be measured once or twice.

C.Calculs：

96 assiette bien à fond plat

DBIL content (μmol / L) =1495.7×(ΔAT-ΔAB)-15.343

Micro glass cuvette

DBIL content (μmol / L) =1099.7×(ΔAT-ΔAB)-10.73

Note:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, it is recommended to dilute the sample before determination.

Exemples:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 assiette bien à fond plat, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol / L) =1495.7×(ΔAT-ΔAB)-15.343=54.95 μmol/L.

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BC5180/BC5185 Total bilirubin (TBIL) Kit d'analyse de contenu