Introduction
- RNA Clean Beads:
- Utilize paramagnetic bead technology for high-throughput purification of RNA or cDNA.
- Designed for in vitro applications such as transcription, antisense RNA (aRNA) amplification, and RNA and cDNA probe synthesis.
- Applications:
- The resulting purified product can be used in various applications, y compris:
- RAP and RT-PCR
- Probes for microarray or macroarray
- RNase protection assays
- Transfection for RNAi experiments
- synthèse d'ADNc and labeling
- The resulting purified product can be used in various applications, y compris:
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen or agencourt AmPure XP) |
| Applications | Advanced applications, such as sequencing, genechip, fluorescence quantification, etc.. |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel ou automatique |
| Échantillon type | RNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Montant de l'échantillon | 20-100µl |
| Récupération | 90% |
| Volume d'élution | ≥20μl |
| Temps de fonctionnement | ≤50 minutes |
Principe
RNA Clean Process:
- Liaison sélective:
- Utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads.
- Élimination des contaminants:
- Excess oligonucleotides, nucléotides, sels, and enzymes are removed through a simple washing procedure.
Avantages
- Récupération élevée – recovery of RNA up to 90%
- Débit élevé – utilisant la technologie de purification par billes magnétiques
- Simple – processus de fonctionnement simplifié, combinaison – lavage – élution
Contenu du kit
| Contenu | BRP-5 | BRP-50 | BRP-500 |
| RNA Clean Beads | 5 ml | 50 ml | 500 ml |
Stockage et stabilité
NE PAS CONGELER.
Commentaires
Il n'y a pas encore de critiques.