Instructions:
- Collection d'échantillons:
- Collect 0.1-1ml blood sample.
- La préparation des échantillons:
- Ajouter 2 à 3 times the volume of 1x red blood cell solution to the blood sample.
- Mix thoroughly by inverting.
- Centrifuger à 12,000 tr/min pour 1 minute.
- Aspirer soigneusement le surnageant. The precipitate should ideally be white or light red.
- Ajouter 400 μl reagent buffer I and 10 μl epoxy resin K (10 mg/ml) to the precipitate.
- Vortex and mix for 15 secondes, then let it sit for 2 minutes.
- Special Case: Low-level Organisms:
- If processing blood from low-level organisms (par exemple., la volaille, birds) containing cell nuclei:
- Add 1x red blood cell solution and directly add 200 μl of reagent buffer I (total volume not exceeding 500 µl).
- Let it sit for 2 minutes.
- Digestion:
- Ajouter 10 μl epoxy resin K (10 mg/ml) au mélange.
- Invert and mix, then digest at 65°C for 10 minutes.
- Pendant cette période, inverser et mélanger 6-7 times to ensure complete digestion.
- Transfer to Reagent Plate:
- Add the solution from the previous step to the 96-well reagent plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
