Introduction
This product is specially designed for stool Extraction d'ARN. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-RAP and Northern hybridization.
Détails
Principe
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Contenu du kit
| Contenu | R662601 | R662602 | R662603 |
| Temps de purification | 48 Préparations | 96 Préparations | 5 X 96 Préparations |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNase I | 600 µl | 2 X 600 µl | 10 X 600 µl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Tampon RW2* | 20 ml | 50 ml | 2 X 100 ml |
| Eau sans RNase | 10 ml | 30 ml | 120 ml |
| 2ml Bead Tubes | 48 | 96 | 5 X 96 |
Stockage et stabilité
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Cependant, stockage à court terme (DNase I up to 1 semaines, MagPure RNA Particles up to 8 semaines) à température ambiante (15–25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être conservés à température ambiante (15–25°C) et sont stables pendant au moins 18 months under these
Commentaires
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