Particules Magpure 100 ml / 400 ml / 1200 ml / 4000 ml

Introduction

• Magnetic bead nucleic acid purification technology utilizes nano or micron superparamagnetic material as the matrix, often using black or yellowish-brown ferric oxide.
• The surface of the beads is coated with functional groups such as carboxyl, hydroxyl, or silicon groups, which facilitate nucleic acid adsorption.
• Silicon-based magnetic beads are the most common, working similarly to classical glass milk or glass fiber filter membrane purification methods.
• Magpure particles are polydisperse silica magnetic beads, comprising 50% ferric oxide at the core and 50% silica on the surface.
• This product serves various purposes including plasmid extraction, gel DNA recovery, product purification, genomic DNA and Extraction d'ARN, as well as viral nucleic acid extraction.

Caractéristiques

Principe

• High salt mediated binding: Magpure particles can selectively recover DNA molecules in solutions containing 2-4M guanidine isothiocyanate, while impurities like protein polysaccharides are not adsorbed.
• Alcohol-mediated binding: Magpure particles can selectively recover DNA/RNA molecules in solutions containing guanidine salt and alcohol (>25%), with proteins and other impurities not being adsorbed.
• After treating biological samples with a digestive solution or lysis buffer, DNA/RNA is released into the reagents from cells, organelles, and protein complexes (ribosomes and nucleosomes).
• Upon adding Magpure particles and binding solution, DNA/RNA adheres to the surface of the particles, forming the DNA/RNA bead complex.
• The magnetic field then separates and collects the magnetic beads, removing impurities such as protein with the waste liquid.
• Following two or three additional cleaning steps, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer.
• DNA/RNA falls off from the surface of the magnetic beads, achieving purification.

Informations de commande

Guide d'achat

• • MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenience of magnetic particles.
  • DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt.
  • Bound DNA is efficiently washed, significantly enhancing DNA purity.
  • High-quality DNA is eluted from the magnetic particles.
  • The automated purification procedure removes enzymes, nucléotides, and other contaminants and inhibitors completely.
  • Purified DNA is ready for direct use in downstream applications, including sequencing and microarray analysis.