Présentation du produit
| Caractéristiques | Caractéristiques |
| Concentration | 100 mg/ml |
| Apparence | Suspension of black particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 1.5-5 µm |
| Conditions de conservation | Température ambiante, valid for up to 2 années.
It is recommended to store at 2-8°C to prevent microbial growth. |
| Magnetic response speed | 15-30 secondes |
| Settling velocity | >5 minutes |
| High salt mediated binding | >2M. guanidine isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride/isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Non-détecté |
| DNA residue | <1 ppm |
| Application recommandée | Extraction de plasmide, gel DNA recovery, genomic DNA extraction et Extraction d'ARN. |
Principe
Magnetic Separation:
- Magnetic beads with silica coating are used.
- After lysing the sample, DNA/RNA is released.
- Magnetic beads and binding solution are added.
- DNA/RNA binds to the surface of the beads.
- A magnet separates the bead-DNA/RNA complex from the solution (impurities).
Liaison sélective:
- High salt-mediated binding (2-4M guanidine isothiocyanate): selectively recovers DNA molecules.
- Alcohol-mediated binding (guanidine salt and >25% alcool): selectively recovers DNA/RNA molecules.
- Proteins and other impurities are not adsorbed to the beads.
Élution:
- The purified DNA/RNA complex is washed to remove contaminants.
- DNA/RNA is released from the beads using sterilized water or TE buffer.
Commentaires
Il n'y a pas encore de critiques.