Fast and Easy Total Purification de l'ADN
- Objectif: Reliable RAP and Southern Blotting
- Caractéristiques:
- Swift purification methods
- Easy-to-follow protocols
- Avantages:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Le sang total
- Plasma
- Sérum
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Cultured cells
- Purifies various types of total DNA:
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ADN total à partir de 200 μl de sang total |
| Applications | RAP, boulon du sud et détection de virus, etc. |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel ou automatique |
| Échantillon type | Le sang total, sang concentré, couche leucocyto-plaquettaire, et d'autres échantillons |
| Montant de l'échantillon | 200µl |
| Volume d'élution | ≥70μl |
| Temps par course | ≤40 minutes |
Principe
- Sample lysis and digestion facilitated by lysate and Protease.
- L'ADN est libéré dans le lysat.
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
Procédure:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: L'ADN est libéré dans le lysat.
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Lavage:
- D'abord, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Élution: DNA is eluted using an Elution Buffer.
Avantages:
-
- High specificity: Selective DNA binding, excluding impurities.
- Efficacité: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Applications:
-
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Avantages
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, comme le sang total, couche leucocyto-plaquettaire, et sang concentré.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Séquençage de deuxième génération (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Contenu du kit
| Contenu | D631001 | D631002 | D631003 |
| Temps de purification | 48 | 96 | 480 |
| Particules MagPure | 1.7 ml | 3.5 ml | 17 ml |
| Protéinase K | 24 mg | 50 mg | 220 mg |
| Tampon de dissolution de protéase | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Buffer DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Tampon d'élution | 15 ml | 30 ml | 100 ml |
Stockage et stabilité
Protéinase K, MagPureParticles should be stored at 2-8°C upon arrival. Cependant, stockage à court terme(jusqu'à 24 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être conservés à température ambiante (15-25°C)et sont stables pendant au moins 18 mois dans ces conditions.
