Introduction
This product offers a fast and reliable method to isolate RNA, y compris les miARN, from a wide range of samples:
- Tissu
- Cellules
- Sang
- Other clinical samples
The extracted RNA is suitable for various downstream applications, y compris:
- RT-RAP (Reverse Transcription PCR)
- Quantitative RT-PCR (qRT-PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| Applications | RT-PCR, synthèse d'ADNc, second-generation sequencing |
| Des produits | ARN, miARN |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Clinical tissues, cellules, lymphocytes |
| Montant de l'échantillon | Tissu: <20 mg
Cellules: <5 X 106 |
| Rendement | 2-50mg |
| Volume d'élution | ≥30 μl |
| Temps par course | ≤25 minutes |
Principe
- Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- Échantillon de lyse et de digestion: The sample lysis process, coupled with proteinase K digestion, takes only 15 minutes, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- Élution: Enfin, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
Avantages
- Efficient DNA removal – One-step Extraction d'ARN can effectively remove genomic DNA
- Haute qualité – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Rapide – the whole extraction only takes 15-25 minutes
- Nontoxic – no toxic phenol-chloroform extraction is required in the extraction
Contenu du kit
| Contenu | DIV4121 |
| Temps de purification | 50 Préparations |
| HiPure DNA Mini Column Ⅱ | 50 |
| Mini-colonnes HiPure ARN | 50 |
| 2Tubes de prélèvement ml | 150 |
| Protéinase K | 24 mg |
| Tampon de dissolution de protéase | 1.8 ml |
| DNase I | 600 µl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Tampon RW2* | 20 ml |
| Eau sans nucléase | 10 ml |
Stockage et stabilité
- Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. Stockage à court terme (jusqu'à 8 semaines) à température ambiante (15–25°C) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. Stockage à court terme (jusqu'à 1 week) à température ambiante (15–25°C) does not affect its performance.
- Remaining Kit Components Storage: Les composants restants du kit peuvent être conservés à température ambiante (15–25°C) et rester stable pour au moins 18 mois dans ces conditions.
Commentaires
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