HiPure Stool DNA Kit

Stool Sample in Molecular Biology Research:

1. Utility and Applications:
   • Stool samples are increasingly utilized in animal molecular genetics, population ecology, behavioral ecology, and intestinal disease diagnosis.
   • They contain gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
2. Défis:
   • Low content of exfoliated cells in the digestive tract and degradation of genetic material pose primary challenges.
   • Presence of inhibitors such as polysaccharides, bile acids, bile salts, bile pigments, digestive juices, and mucus can affect RAP-based molecular analysis.

Importance de l'extraction de l'ADN:

Critical Role:

• • Selecting appropriate extraction methods is crucial for obtaining high-quality DNA and ensuring successful downstream detection of stool DNA.

HiPure Stool DNA Kit:

1. Solution Provided:
   • The HiPure Stool DNA Kit from Magen Company offers a promising solution.
   • Features good universality, high cost-effectiveness, high yield, and purification.
2. Unique Features:
   • Utilizes a unique solution system and inhibitory factor adsorbent to efficiently remove various impurities in stool samples.
   • The purified DNA can be directly used for PCR, PCR quantitative, et d'autres applications.
3. Efficiency and Ease of Use:
   • Enables rapid and reliable isolation of high-quality genomic DNA from various stool samples.
   • Capable de traiter jusqu'à 100 mg of stool samples within 60 minutes.
   • Combines reversible nucleic acid binding properties with colonne de rotation technologie, eliminating PCR inhibiting compounds.
   • Suitable for PCR, digestion des restrictions, et applications de séquençage de nouvelle génération.
4. Sustainability and Scalability:
   • Eliminates the need for organic extractions, reducing plastic waste and hands-on time.
   • Allows multiple samples to be processed in parallel, enhancing efficiency.

Détails

Caractéristiques

Caractéristiques	Caractéristiques
Fonctions principales	Isolation total DNA from 50-100mg stool samples
Applications	RAP, Blot du Sud, enzyme digestion and NGS, etc..
Méthode de purification	Mini colonne d'essorage
Technologie de purification	Technologie de la silice
Méthode de processus	Manuel (centrifugation ou vide)
Échantillon type	Tabouret
Montant de l'échantillon	50-100mg
Rendement	3-15µg
Volume d'élution	≥30 μl
Temps par course	≤60 minutes
Volume de liquide transporté par colonne	750µl
Rendement de liaison de la colonne	100µg

Principe

Stool DNA Extraction Process:

1. Homogénéisation et lyse:
   • Stool sample is homogenized and treated in a specially formulated buffer containing detergent.
   • This step effectively lyses bacteria, levure, and fungal samples present in the stool.
2. Élimination des contaminants:
   • Our proprietary Absorber Solution is used to remove humic acid, protéines, polysaccharides, et d'autres contaminants de l'échantillon lysé.
3. Reliure et lavage:
   • Adjusted binding conditions facilitate the attachment of DNA to a DNA Mini Column.
   • Two rapid wash steps are performed to remove trace contaminants, ensuring purity of the DNA.
4. Elution of Purified DNA:
   • Pure DNA is eluted from the column in a low ionic strength buffer.
   • The resulting purified DNA is ready for direct use in downstream applications without the need for further purification.

Avantages

• Haute pureté – un adsorbant unique peut éliminer complètement les facteurs inhibiteurs
• Haute concentration – maximum extraction of total DNA from stool samples
• Récupération élevée – L'ADN peut être récupéré au niveau du PG
• Bonne répétabilité – la technologie de la silice peut obtenir des résultats idéaux à chaque fois

Contenu du kit

Contenu	D314102	D314103
Temps de purification	50 Préparations	250 Préparations
HiPure DNA Mini Colonnes II	50	250
2Tubes de prélèvement ml	50	250
2ml Bead Tubes	50	250
Protéinase K	24 mg	120 mg
Tampon de dissolution de protéase	1.8 ml	10 ml
Buffer SPL	40 ml	200 ml
Tampon PCI	40 ml	200 ml
Tampon AL	20 ml	80 ml
Tampon GW1	22 ml	88 ml
Tampon GW2	20 ml	2 X 50 ml
Tampon AE	15 ml	30 ml

Stockage et stabilité

Instructions de stockage:

1. Proteinase K and Buffer PCI:
   • À l'arrivée, store at 2-8°C.
   • Stockage à court terme (jusqu'à 12 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances.
2. Composants du kit restant:
   • Stocker à température ambiante (15-25°C).
   • Stable pour au moins 18 mois dans ces conditions.
3. Entire Kit:
   • Can be stored at 2–8°C.
   • Buffers should be redissolved before use if stored at this temperature.
   • Ensure all buffers are at room temperature when used.