Introduction
- HiPure ADN plasmidique Plus 96 Trousse:
- Enables up to 96 mini-preps to be performed simultaneously.
- Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
- Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
- Principales fonctionnalités:
- Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
- Plasmid DNA purified with the kit is immediately ready for use.
- Eliminates the need for phenol extraction and ethanol precipitation.
- High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement jusqu'à 20 µg d'ADN plasmidique à partir de 1,5 ml de culture bactérienne en utilisant 96 bien lier la plaque et 96 filterplate |
| Applications | Enzyme digestion, séquençage, RAP, labeling, etc.. |
| Méthode de purification | 96 bien plaque |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Conventional plasmid, plasmid less than 30KB |
| Montant de l'échantillon | 1-1.5ml（x96） |
| Rendement | 1-15µg/1ml |
| Volume d'élution | ≥70μl |
| Temps par course | ≤60 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 70µg |
Principe
- HiPure Plasmid Procedure:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
- Procedure Steps:
- Preparation and Clearing of Bacterial Lysate:
- The alkaline method is used to prepare and clear bacterial lysate.
- DNA Binding:
- Supernatant transferred to a column to bind DNA to the silica membrane.
- Washing and Elution:
- Proteins and other impurities washed away.
- L'acide nucléique a finalement été élué avec un tampon à faible teneur en sel (10mmTris, pH9,0, 0.5mmEDTA).
- Preparation and Clearing of Bacterial Lysate:
Avantages
- Haute pureté – purified plasmid can be directly used in sequencing, enzyme digestion PCR, etc..
- Rapide – it takes only 60 minutes to complete the isolation
- Haut rendement – up to 1mg plasmid can be bound in one column
- Économie – high cost-performance
Contenu du kit
| Contenu | P100601 | P100602 | P100603 |
| Temps de purification | 1 X 96 Préparations | 4 X 96 Préparations | 20 X 96 Préparations |
| RNase A | 5 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 600 ml |
| Buffer P2 | 30 ml | 120 ml | 600 ml |
| Buffer P3 | 40 ml | 180 ml | 800 ml |
| Buffer PW1 | 100 ml | 500 ml | 2 X 1000 ml |
| Buffer PW2 | 50 ml | 2 X 100 ml | 4 X 200 ml |
| Tampon d'élution | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Stockage et stabilité
The kit components can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C.
Guide d'achat
| Nom | CAT NO | Montant de l'échantillon | Column type | Volume d'élution | Rendement | Temps par course | Centrifuge requirements |
| Mini kit plasmidique HiPure | P1001 | 1-5ml | 1.5ml column | 30-50µl | 5–35µg/5ml | ≤ 30 minutes | small centrifuge |
| HiPure Plasmide Mini Kit II | P1002 | 5-15ml | 1.5ml column | 75-100µl | 20-80µg/15ml | ≤ 30 minutes | small centrifuge |
| HiPure Plasmide Plus 96 Trousse | P1006 | 1-1.5ml | 96 bien plaque | 70-150µl | 1-15μg/1ml | ≤60 minutes | 96-well plate centrifuge |
| Kit Midi Plasmide HiPure Fastfilter | P1013 | 30-50ml | 15ml column | 0.3-0.8ml | 50–250µg/50ml | ≤60 minutes | 15ml centrifuge |
| Kit Maxi Plasmide HiPure Fastfilter | P1014 | 100-200ml | 50ml column | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minutes | 50ml centrifuge |
Commentaires
Il n'y a pas encore de critiques.