Introduction
The HiPure Mini system provides a fast, simple, and cost-effective ADN plasmidique miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture |
| Applications | Enzyme digestion, séquençage, RAP, clonage, etc.. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Conventional plasmid, plasmid less than 30KB |
| Montant de l'échantillon | High copy plasmid: 1-5ml culture medium
Low copy plasmid : 5-10ml culture medium |
| Rendement | 20-80µg |
| Volume d'élution | ≥75μl |
| Temps par course | Complete 1-24 samples in 30 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 70µg |
Principe
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. Après avoir lavé les protéines et autres impuretés, l'acide nucléique a finalement été élué avec un tampon à faible teneur en sel (10mmTris, pH9,0, 0.5mmEDTA).
Avantages
- Haute pureté – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc..
- Rapide – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
- Haut rendement – up to 70µg plasmid can be binded in one column
Contenu du kit
| Contenu | P100202 | P100203 |
| Temps de purification | 50 Préparations | 250 Préparations |
| RNase A | 5 mg | 20 mg |
| Buffer P1 | 30 ml | 140 ml |
| Buffer P2 | 30 ml | 140 ml |
| Buffer P3 | 40 ml | 200 ml |
| Buffer PW1 | 30 ml | 140 ml |
| Buffer PW2* | 12 ml | 50 ml |
| Tampon d'élution | 15 ml | 30 ml |
| HiPure DNA Mini Columns III | 50 | 250 ml |
| 2 Tubes de prélèvement ml | 50 | 250 ml |
Stockage et stabilité
The kit components can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
Guide d'achat
| Nom | CAT NO | Montant de l'échantillon | Column type | Elutio volume | Rendement | Temps par course | Centrifuge requirements |
| Mini kit plasmidique HiPure | P1001 | 1-5ml | 1.5ml column | 30-50µl | 5–35µg/5ml | ≤ 30 minutes | small centrifuge |
| HiPure Plasmide Mini Kit II | P1002 | 5-15ml | 1.5ml column | 75-100µl | 20-80µg/15ml | ≤ 30 minutes | small centrifuge |
| HiPure Plasmide Plus 96 Trousse | P1006 | 1-1.5ml | 96 bien plaque | 70-150µl | 1-15μg/1ml | ≤60 minutes | 96 well plate centrifuge |
| Kit Midi Plasmide HiPure Fastfilter | P1013 | 30-50ml | 15ml column | 0.3-0.8ml | 50–250µg/50ml | ≤60 minutes | 15ml centrifuge |
| Kit Maxi Plasmide HiPure Fastfilter | P1014 | 100-200ml | 50ml column | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minutes | 50ml centrifuge |
Commentaires
Il n'y a pas encore de critiques.