Mini kit plasmidique HiPure

Introduction

• Rapide, Simple, and Cost-Effective: Offers a rapid, easy-to-use, and economical method for ADN plasmidique miniprep suitable for routine molecular biology applications.
• Silica Membrane Technology: Utilizes silica membrane technology, eliminating the need for cumbersome steps associated with loose resins or slurries, streamlining the purification process.
• Immediate Usability: Purified plasmid DNA is immediately ready for downstream applications, saving time and effort.
• No Phenol Extraction or Ethanol Precipitation: Bypasses the need for phenol extraction and ethanol precipitation, simplifying the workflow and reducing hands-on time.
• ADN de haute qualité: Yields high-quality plasmid DNA eluted in a small volume of Tris buffer or water, ensuring optimal performance in downstream applications.

Caractéristiques

Caractéristiques	Caractéristiques
Fonctions principales	Isolation of up to 35μg plasmid DNA from 1-5ml bacterial culture
Applications	Digestion enzymatique, séquençage, RAP, clonage, etc..
Méthode de purification	Mini colonne de rotation
Technologie de purification	Technologie de la silice
Méthode de processus	Manuel (centrifugation ou vide)
Échantillon type	Plasmide conventionnel, plasmide de moins de 30 Ko
Montant de l'échantillon	Plasmide à copie élevée: 1-5ml culture medium Low copy number plasmid: 5-10ml de milieu de culture
Rendement	5-35µg
Volume d'élution	≥30 μl
Temps par course	Complet 1-24 des échantillons dans 30 minutes
Volume de liquide transporté par colonne	800µl
Rendement de liaison de la colonne	35µg

Principe

• Lyse alcaline: Begins with the alkaline lysis of bacterial cells, which breaks down the cell membrane and releases plasmid DNA into the lysate.
• Silica-Based DNA Binding: The released DNA is adsorbed onto the silica membrane in the presence of high salt, facilitating efficient binding of DNA to the membrane surface.
• Three Basic Steps: The procedure involves three main steps: (1) Preparation and clearing of the bacterial lysate using an alkaline method, (2) Transfer of the cleared lysate to a column for DNA binding, et (3) Washing to remove proteins and other impurities.
• Silica Membrane: Utilizes a unique silica membrane in the kit, which completely replaces traditional glass or silica slurries typically used in plasmid DNA minipreps.
• Elution with Low-Salt Buffer: After washing to remove impurities, nucleic acid, including plasmid DNA, is eluted from the silica membrane using a low-salt buffer containing 10mM Tris, pH 9.0, and 0.5mM EDTA.

Avantages

• Haute pureté – le plasmide purifié peut être directement utilisé dans le séquençage, enzyme digestion PCR, etc..
• Rapide – ça prend seulement 1 minute to obtain supernatant by optimized solution (10 minutes pour d'autres marques)
• Haut rendement – up to 35µg plasmid can be bound in one column

Contenu du kit

Contenu	P100102	P100103
Temps de purification	100 Préparations	250 Préparations
RNase A	5 mg	10 mg
Tampon P1	30 ml	80 ml
Tampon P2	30 ml	80 ml
Tampon P3	40 ml	100 ml
Tampon PW1	60 ml	140 ml
Tampon PW2*	20 ml	50 ml
Tampon d'élution	15 ml	30 ml
HiPure DNA Mini Colonnes II	100	250
2 Tubes de prélèvement ml	100	250

Stockage et stabilité

• Conditions de stockage: Kit components can be stored dry at room temperature (15-25°C) and remain stable for a minimum of 18 mois dans ces conditions.
• Buffer Precipitates: In case of any precipitates forming in the buffers, warm them at 37°C to dissolve effectively.
• Buffer P1 Stability: After the addition of RNase A, Buffer P1 remains stable for up to 6 mois lorsqu'il est conservé à 2-8°C.

Données d'expérimentation

Guide d'achat

Nom	N° DE CHAT	Montant de l'échantillon	Type de colonne	Volume d'élution	Rendement	Temps par course	Exigences de la centrifugeuse
Mini kit plasmidique HiPure	P1001	1-5ml	1.5colonne ml	30-50µl	5–35µg/5ml	≤ 30 minutes	petite centrifugeuse
HiPure Plasmide Mini Kit II	P1002	5-15ml	1.5colonne ml	75-100µl	20-80µg/15ml	≤ 30 minutes	petite centrifugeuse
HiPure Plasmide Plus 96 Trousse	P1006	1-1.5ml	96 bien plaque	70-150µl	1-15µg/1ml	≤60 minutes	96-centrifugeuse à plaque à puits
Kit Midi Plasmide HiPure Fastfilter	P1013	30-50ml	15colonne ml	0.3-0.8ml	50–250µg/50ml	≤60 minutes	15ml centrifugeuse
Kit Maxi Plasmide HiPure Fastfilter	P1014	100-200ml	50colonne ml	0.5-1.5ml	0.4–1mg/200ml	≤60 minutes	50ml centrifugeuse