This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-RAP, Northern Blot, poly A+ purification, nucleic acid protection, et traduction in vitro.
Détails
Contenu du kit
| Contenu | R415002 | D415003 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure ARN | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 500 |
| TCEP (1M.) | 0.29 g | 5 X 0.29 g |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Tampon RW1 | 50 ml | 250 ml |
| Tampon RW2* | 20 ml | 2 X 50 ml |
| Eau sans RNase | 10 ml | 30 ml |
Stockage et stabilité
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
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