Introduction
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cellule, tissus, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-RAP, Transfert du Nord, ARN poly A+ (ARNm) purification, protection contre les nucléases, et traduction in vitro.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from <1g simple plant sample (including plant sample containing starch) without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Méthode de purification | Midi colonne de rotation |
| Technologie de purification | Technologie de la silice, DNA filtration technology |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Plant leaves, roots, stems, fruits, fungi, etc.. |
| Montant de l'échantillon | 0.5-1 g |
| Volume d'élution | ≥400μl |
| Temps par course | ≤40 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 1mg |
Principe
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Avantages
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Haute qualité – l'ARN total de haute pureté peut être directement utilisé dans diverses applications sensibles en aval
- Rapide – medium isolation of several samples can be completed in 40 minutes
- Sûr – no phenol chloroform extraction required
- Sensible – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Contenu du kit
| Contenu | R415202 | D415203 |
| Temps de purification | 20 Préparations | 100 Préparations |
| HiPure DNA Midi Column II | 20 | 100 |
| HiPure RNA Midi Columns II | 20 | 100 |
| 15Tubes de prélèvement ml | 40 | 200 |
| Buffer RLC | 120 ml | 550 ml |
| Buffer PRC1 | 120 ml | 550 ml |
| Tampon RW1 | 70 ml | 400 ml |
| Tampon RW2* | 20 ml | 100 ml |
| Eau sans RNase | 30 ml | 120 ml |
Stockage et stabilité
The Kit can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés. Pendant l'expédition, crystals or precipitation may form in the Buffer RLC/PRC1. Dissoudre en réchauffant le tampon à 37°C.
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