Introduction
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-RAP, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from bacteria, yeast cells |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Bactéries, yeast cells |
| Montant de l'échantillon | Bactéries: <10^ 9；Yeast cells：<2 x10^7 |
| Volume d'élution | ≥30 μl |
| Temps par course | ≤40 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (tel que guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Enfin, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Avantages
- Rapide – Plusieurs échantillons peuvent être extraits dans 30 minutes
- Haute pureté – the purified RNA can be directly used in various downstream applications
- Récupération élevée – RNA can be recovered at the level of PG
- Bonne répétabilité – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried wall-breaking enzymes and glass beads
Contenu du kit
| Contenu | R418202 | R418203 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure ARN | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| Glass Beads (0.1-0.6mm) | 30 g | 150 g |
| DNase I | 600 µl | 5 X 600 µl |
| DNase Buffer | 6 ml | 30 ml |
| Tampon de dissolution de protéase | 1.8 ml | 15 ml |
| Tampon ATL | 50 ml | 200 ml |
| Buffer PHC | 50 ml | 200 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Tampon RW1 | 50 ml | 250 ml |
| Tampon RW2* | 20 ml | 2 X 50 ml |
| Eau sans RNase | 10 ml | 30 ml |
Stockage et stabilité
Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Cependant, stockage à court terme (DNase I up to 1 semaines, Buffer PHC up to 12 semaines) à température ambiante(15–25°C) n'affecte pas leurs performances. The remaining kit components can be stored at roomtemperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions.
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