Especificación
| Atributo | Detalles |
|---|---|
| Almacenamiento | 2-8℃ |
| Unidad | Caja |
| Período de validez | 6 meses |
| Nombre en inglés | Pepsase Activity Assay Kit |
| Método de detección | Lector de espectrofotómetro/microplato |
| Especificación | 100T/48S |
Composición de productos
| Nombre del reactivo | Especificación | Condición de almacenamiento |
|---|---|---|
| Solución de extracción | Líquido 50 mL×1 bottle | Almacenar a 4 ℃ |
| reactivo uno | Powder×1 bottle | Almacenar a 4 ℃ |
| Reactivo dos | Líquido 15 mL×1 bottle | Almacenar a 4 ℃ |
| Reactivo tres | Powder×1 bottle | Almacenar a 4 ℃ |
Instructions for preparing the solutions:
- Solution One: Agregar 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: Agregar 10 mL of distilled water before use and dissolve completely.
Descripción del Producto:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- Agua destilada
Nota: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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