Descripción del producto
- ditiotreitol (TDT), or Cleland’s reagent, Es un agente reductor orgánico de molécula pequeña..
- In its reduced state, DTT exists as a linear molecule, but upon oxidation, it forms a six-membered ring structure containing disulfide bonds.
- DTT is a potent reducing agent, largely due to the stability of its oxidized state, the six-membered ring with disulfide bonds.
- One application of DTT is as a reducing agent and deprotectant for thiol-modified DNA.
- Thiol-modified DNA tends to dimerize in solution, especialmente en presencia de oxígeno, which reduces the efficiency of certain coupling reactions, such as DNA immobilization in biosensors.
- The addition of DTT to DNA solutions can reduce dimerization, enhancing experimental efficiency.
- DTT is also commonly used to reduce disulfide bonds in proteins, preventing the formation of intra- or intermolecular disulfide bonds between cysteine residues.
- Sin embargo, DTT may not effectively reduce disulfide bonds buried within the protein structure (inaccessible solvent), which often requires denaturation of the protein.
- The rate of disulfide bond reduction in the presence of DTT can indicate the depth of burial of these bonds, allowing insights into protein folding.
- Other names: 1,4-Dithio-DL-threitol, Threo-1,4-dimercapto-2, 3-butandiol
Reseñas de los clientes sobre los productos Solarbio como referencia
| Atributo | Value |
|---|---|
| CAS | 3483-12-3 |
| Especificación | 1gramo ; 5gramo ; 25gramo ; 100gramo ; 10gramo |
| Fórmula molecular | C4H10O2S2 |
| Peso molecular | 154.25 |
| Apariencia | polvo blanco |
| Condiciones de almacenaje | 2-8°C |
| Expiry | 4 años |
| Pureza | 99.5% |
| Unidad | Botella |
| Calificación | Grado de biotecnología |
| Solubilidad | 100mg/ml in Water |
To be noticed
- Due to its susceptibility to air oxidation, DTT has poor stability; sin embargo, its lifespan can be extended by freezing or handling under inert gas.
- Our biochemical reagents are typically non-sterile unless otherwise specified; if used for cell experiments, please perform appropriate pretreatment.
- Product information is for reference only; for inquiries, please call 400-968-6088.
- This product is for research use only. Do not use for pharmaceutical, clinical diagnostic, or therapeutic purposes, or food, cosmetic, or household purposes.
- Por tu seguridad y salud, please wear lab coats and disposable gloves and masks during operation.
Reseñas
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