Bilirrubina Directa Solarbio (DBIL) Kit de ensayo 50T | 48S

Nota: Tome dos o tres muestras diferentes para predecir antes de la prueba..

Equipo de operación: Lector de espectrofotómetro/microplato

Numero de catalogo: BC5175

Tamaño: 50t | 48S

Componentes:

reactivo yo: 25 mL×1. Almacene a 2-8 ℃.

Reactivo II: 6 mL×1. Almacene a 2-8 ℃.

Descripción del Producto：

Bilirrubina directa (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 Nuevo Méjico. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 Nuevo Méjico.

Reactivos y equipos necesarios pero no suministrados：

Lector de espectrofotómetro/microplato, centrífuga de escritorio, constant temperature foster box/water bath, pipette, cubeta de microvidrio/placa de fondo plano de 96 pocillos, hielo, y agua destilada.

Procedimiento

A. preparación de la muestra:

Suero, plasma, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

B.Determinación procedimiento：

1. Preheat the spectrophotometer/microplate reader for 30min, ajustar la longitud de onda a 450 Nuevo Méjico, and set the spectrophotometer counter to zero with distilled
2. Determinación:

Reactivo (µL)	Tubo de ensayo	tubo en blanco
Muestra	8	–
Agua destilada	–	8
reactivo yo	192	192
Mezclar bien. React at 37℃ for 5 minutos. Mida la absorbancia en 450 Nuevo Méjico, and record it as A1T, A1B;
Reactivo II	48	48
Mezclar bien. React at 37℃ for 5 minutos. Mida la absorbancia en 450 Nuevo Méjico, and record it as A2T, A2B. Calculate the ΔAT =A1T-A2T and the ΔAB =A1B-A2B. Blank tubes only need to be measured once or twice.

C.Cálculos：

96 well flat-bottom plate

DBIL content (μmol/L) =1495.7×(Δat-ΔAb)-15.343

Micro glass cuvette

DBIL content (μmol/L) =1099.7×(Δat-ΔAb)-10.73

Nota:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, Se recomienda diluir la muestra antes de la determinación..

Ejemplos:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 well flat-bottom plate, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol/L) =1495.7×(Δat-ΔAb)-15.343=54.95 μmol/L.

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