Introducción
This product is specially designed for stool extracción de ARN. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Detalles
Principio
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Contenido del kit
| Contenido | R662601 | R662602 | R662603 |
| Tiempos de purificación | 48 preparativos | 96 preparativos | 5 X 96 preparativos |
| Partículas de ARN MagPure | 1.7 ml | 4.0 ml | 18 ml |
| ADNasa I | 600 µl | 2 X 600 µl | 10 X 600 µl |
| Tampón ADNasa | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| MCB tampón* | 18 ml | 30 ml | 150 ml |
| Tampón MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Búfer RW2* | 20 ml | 50 ml | 2 X 100 ml |
| Agua libre de ARNasa | 10 ml | 30 ml | 120 ml |
| 2ML tubos de cuentas | 48 | 96 | 5 X 96 |
Almacenamiento y estabilidad
MagPure RNA Particles should be stored at 2–8°C upon arrival. La ADNasa I debe conservarse a -20°C.. Sin embargo, almacenamiento a corto plazo (ADNasa I hasta 1 semanas, MagPure RNA Particles up to 8 semanas) a temperatura ambiente (15–25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar a temperatura ambiente. (15–25°C) y son estables durante al menos 18 months under these
Reseñas
Aún no hay reseñas.