Fast and Easy Total Purificación de ADN
- Objetivo: Reliable PCR and Southern Blotting
- Características:
- Swift purification methods
- Easy-to-follow protocols
- Beneficios:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Sangre pura
- Plasma
- Suero
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Células cultivadas
- Purifies various types of total DNA:
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation of total DNA from 200μl whole blood |
| Aplicaciones | PCR, Detección de virus y pernos del sur., etc. |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | Sangre pura, sangre concentrada, capa leucocitaria, y otras muestras |
| Cantidad de muestra | 200µl |
| Volumen de elución | ≥70μl |
| Tiempo por carrera | ≤40 minutos |
Principio
- Sample lysis and digestion facilitated by lysate and Protease.
- El ADN se libera en el lisado..
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
Procedimiento:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: El ADN se libera en el lisado..
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Lavado:
- Primero, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Elución: DNA is eluted using an Elution Buffer.
Ventajas:
-
- High specificity: Selective DNA binding, excluding impurities.
- Eficiencia: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Aplicaciones:
-
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Ventajas
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, como la sangre entera, capa leucocitaria, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Secuenciación de segunda generación (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Contenido del kit
| Contenido | D631001 | D631002 | D631003 |
| Tiempos de purificación | 48 | 96 | 480 |
| Partículas MagPure | 1.7 ml | 3.5 ml | 17 ml |
| Proteinasa K | 24 mg | 50 mg | 220 mg |
| Tampón de disolución de proteasa | 1.8 ml | 3 ml | 15 ml |
| MLA de búfer | 30 ml | 60 ml | 270 ml |
| Memoria intermedia DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Búfer bw3 | 30 ml | 60 ml | 270 ml |
| Tampón de elución | 15 ml | 30 ml | 100 ml |
Almacenamiento y estabilidad
Proteinasa K, Las partículas de los maíz deben almacenarse a 2-8 ° C a la llegada. Sin embargo, almacenamiento a corto plazo(hasta 24 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento. Los componentes restantes del kit se pueden almacenar a temperatura ambiente. (15-25°C)y son estables durante al menos 18 meses en estas condiciones.
