Introducción del producto
This product offers a rapid and efficient method to isolate DNA from a wide range of samples, incluido:
- Tejidos
- Células
- Sangre
- Saliva
- Swabs
- Blood Spots
- Semen
- And more!
The extracted DNA is ready for immediate use in downstream applications like:
- PCR (Reacción en cadena de la polimerasa)
- qPCR (PCR cuantitativa)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
Especificación
| Características | Especificaciones |
| Funciones principales | Isolation of total DNA from tissue/blood /body fluid /swab /dry blood spots |
| Aplicaciones | PCR,qPCR, Detección de virus y pernos del sur., etc.. |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Tejido, células, sangre, saliva, hisopos, manchas de sangre, semen, y otras muestras clínicas |
| Cantidad de muestra | Tejido sólido:1-10mg, Sangre anticoagulante:200metro |
| Producir | 1 -15µg |
| Volumen de elución | ≥20μl |
| Tiempo por carrera | 30 -60 minutos |
| Volumen de transporte de líquido por columna | 750µl |
| Rendimiento de unión de la columna | 100µg |
Principio
- Lisis y digestión: Begin by lysing and digesting the sample using a specialized lysate and protease combination. This step facilitates the release of DNA into the lysate, preparing it for the purification process.
- Adsorption Column: Transfer the lysate mixture to an adsorption column. Aquí, the nucleic acid selectively adheres to the silica membrane, while proteins and other contaminants remain unadsorbed. This selective binding ensures the efficient removal of impurities.
- Filtration: Employ filtration to efficiently remove the unadsorbed proteins and other contaminants from the mixture. This step further enhances the purity of the nucleic acid sample.
- Thorough Washing: Perform a thorough washing step to eliminate any remaining proteins and impurities, ensuring the purity of the nucleic acid sample.
- Elución: Finalmente, elute the purified nucleic acid from the adsorption column using a low-salt buffer solution (10mm tris, pH 9.0, 0.5mmEDTA). This gentle elution process preserves the integrity of the DNA, readying it for downstream applications.
Ventajas
- Versatilidad: Suitable for a diverse range of downstream applications, incluyendo PCR, qPCR, digestión enzimática, hibridación, y más. Whether you’re performing basic research or advanced molecular assays, our DNA meets your requirements.
- Eficiencia: Accelerate your workflow with fast DNA extraction. Say goodbye to time-consuming processes like leukocyte separation, extracción orgánica, o precipitación de etanol. Our method streamlines the extraction process for swift results.
- Simplicity: Enjoy a straightforward extraction process. No complex steps or specialized equipment are required. Obtain all nucleic acids directly through simple digestion, saving you time and effort in the lab.
- Aplicabilidad amplia: Our DNA extraction method is versatile and adaptable. It can handle various sample types, including liquid samples, tejidos animales, y células cultivadas. Whether you’re working with blood, tejidos, or cell cultures, our method delivers consistent results across different sample types.
Contenido del kit
| Contenido | VD3018 |
| Tiempos de purificación | 100 |
| 2Tubos de recolección de ml | 2×100 |
| ATL de búfer | 60 ml |
| Amortiguador AL | 60 metro |
| Memoria intermedia GW1 | 44 metro |
| Búfer GW2 | 50 metro |
| Proteinasa K | 60 mg |
| Tampón de disolución de proteasa | 5 ml |
| Buffer AF | 15 metro |
Almacenamiento y estabilidad
- Proteinase K Storage:
- A llegado, almacenar a 2-8 ° C.
- Almacenamiento a corto plazo (hasta 12 semanas) a temperatura ambiente (15-25°C) es aceptable sin afectar el rendimiento.
- Componentes del kit restantes:
- Store at room temperature (15-25°C).
- Estable por al menos 18 meses en estas condiciones.
- Entire Kit Storage:
- Can be stored at 2-8°C.
- Ensure buffers are redissolved before use if stored at this temperature.
- Buffers should be at room temperature when used.
Datos del experimento
Reseñas
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