HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, secuenciación, digestión de restricción, or various labeling reactions. Además, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Recover DNA fragments between 60bp-10kbp from agarose gel |
| Aplicaciones | PCR, NGS, labeling, ligation and enzyme digestion, etc.. |
| Método de purificación | Mini columna de giro |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Agarose gel, PCR products, enzyme products |
| Cantidad de muestra | Agarose gel: ≤500mg |
| Recuperación | ≥80% |
| Volumen de elución | ≥15 μl |
| ≤20 minutes（1-24 samples） | |
| Volumen de transporte de líquido por columna | 800l |
| Rendimiento de unión de la columna | 35µg |
Principio
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Ventajas
- High recovery efficiency – ≥80% DNA recovery
- General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
- Fast – isolation can be completed in 10-15 minutes by column gel method
Contenido del kit
| Contenido | D211102 | D211103 |
| Tiempos de purificación | 100 preparativos | 250 preparativos |
| PIB de búfer | 120 ml | 250 ml |
| Buffer DW2 | 50 ml | 2 X 50 ml |
| Tampón de elución | 20 ml | 30 ml |
| Minicolumnas HiPure DNA II | 100 | 250 |
| 2 Tubos de recolección de ml | 100 | 250 |
Almacenamiento y estabilidad
The Kit should be stored dry at room temperature (15-25°C) y son estables durante al menos 18 meses en estas condiciones. Si se forma algún precipitado en los tampones, calentar a 37 ℃ para disolver.
Datos del experimento
Reseñas
Aún no hay reseñas.