This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hibridación, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation both Circulating DNA/RNA (incluir miARN) de 1-5 ml serum and plasma |
| Aplicaciones | qPCR / RT-PCR, liquid or solid-phasechip analysis, hibridación y detección de SNP |
| Método de purificación | midi columna de giro |
| Tecnología de purificación | tecnología de sílice, Tecnología de filtración de ADN. |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | suero, plasma, and other cell-free liquid samples |
| Cantidad de muestra | 1-5 ml |
| Volumen de elución | ≥20μl |
| Tiempo por carrera | ≤100 minutes |
| Volumen de transporte de líquido por columna | 4 ml |
| Rendimiento de unión de la columna | 1 mg |
Principio
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. ADN / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finalmente, ADN / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hibridación y detección de SNP.
Ventajas
- Alto rendimiento – most optimized process to obtain maximum free RNA and small RNA
- Alta concentración – Volumen de elución bajo (>20µl) to ensure nucleic acid concentration
- Alta pureza – low alcohol combination, completely remove inhibitor and protein pollution
- Alta recuperación – silica gel column technology can recover nucleic acid molecules at the level of PG
- Large volume – 1-2ml serum and plasma samples can be processed at one time
Contenido del kit
| Contenido | R431602 | D431603 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| Hipure ARN Micro Columnas | 50 | 5 X 50 |
| HiPure Viral Midi Columns | 50 | 5 X 50 |
| 15 Tubos de recolección de ml | 50 | 5 X 50 |
| 2Tubos de recolección de ml | 50 | 5 X 50 |
| Buffer CFL | 150 ml | 2 X 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 X 250 ml |
| Búfer RW2* | 2 X 50 ml | 5 X 100 ml |
| Agua libre de ARNasa | 10 ml | 50 ml |
Almacenamiento y estabilidad
The kit components can be stored at room temperature (15–25°C) y son estables durante al menos 18 meses en estas condiciones.
Reseñas
Aún no hay reseñas.