Nukleinsäure-Testkit für das White-Spot-Syndrom-Virus (WSSV). (PCR-Fluoreszenzsonden-Methode) 24T

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Es wird innerhalb von eintreffen 7-10 Tage. Die Versandkosten sind im Produktpreis enthalten.

Produkteinführung：

This test kit offers a rapid and accurate method for detecting White Spot Syndrome Virus (WSSV) in shrimp.

• Technologie: Employs real-time fluorescent quantitative PCR (TaqMan probe method) for sensitive and specific detection.
• Ziele: Utilizes specific primers and fluorescent probes designed to target the unique nucleic acid sequence of WSSV.
• Anwendungen: Detects WSSV in various shrimp samples, einschließlich:
  • Animal tissues
  • Feed
  • Other sample types (consult kit specifications)
• Dual Detection: Provides qualitative and quantitative results:
  • Qualitative: Determines the presence or absence of WSSV.
  • Quantitative: Measures the amount of WSSV nucleic acid present, allowing for assessment of viral load.
• Real-Time Monitoring: Utilizes real-time PCR for continuous monitoring of amplification throughout the reaction, enabling rapid results.

This user-friendly test kit provides a valuable tool for shrimp farmers, researchers, and diagnostic labs for effective WSSV detection and management.

Produktinhalte：

Komponenten	WP2101005-01（24T）
WSSV reaction solution	23μL per well × 8 wells per strip × 3 Streifen
WSSV positive control	100μL
Negativkontrolle	100μL

Lagerung:

Store at -20°C, with a shelf life of at least 12 Monate.

Applicable instrument：

ABI series (ABI 7300/ABI 7500/Step One, usw.), Roche LightCycler series, Bio-Rad CFX 96, SanshiBio Q162D, and other real-time fluorescence quantitative PCR instruments

Probenverarbeitung：

Process the samples according to relevant standards, and store the processed samples for later use.

Experimental procedures：

• Probenvorbereitung (Probenvorbereitungsbereich)

Please refer to the instruction manual of the total DNA/RNA Extraction Kit, or use the ES08 fully automated nucleic acid extraction and purification instrument with a matching rapid nucleic acid extraction kit (magnetic bead method), or other nucleic acid extraction kits/methods that comply with relevant standards to extract nucleic acid from the processed samples. The extracted sample nucleic acid should be tested as soon as possible and stored in an icebox, or else stored at -20°C.

• Vorbereitung des Reaktionssystems (Adding Area)

2.1 Take out the WSSV positive control tube, negative control tube, and reaction tubes containing the WSSV reaction solution required for the experiment (n+2) (d.h., n samples to be tested + Positive Kontrolle + Negativkontrolle), completely thaw the reagents at room temperature, Zentrifuge für 10 Sekunden, centrifuge the liquid from the tube wall and lid to the bottom of the tube, and store in an icebox for later use.

2.2 Then add 2μL of extracted negative control, sample nucleic acid, and WSSV positive control to the reaction tube in the eight-row strip, close the tube lid, eine Aufnahme machen, and make the total volume of each reaction 25μL. Gründlich mischen, Zentrifuge für 30 Sekunden, and perform the amplification experiment on the real-time fluorescence quantitative PCR instrument.

• Fluorescent PCR Amplification (Bereich Amplifikation und Produktanalyse)

Vordenaturierung bei 95°C für 3 Protokoll; denaturation at 95°C for 5 Sekunden, Glühen und Streckung bei 60°C für 30 Sekunden, für 40 Fahrräder, collect fluorescence signal at 60°C. Choose FAM as the fluorescence group, and None as the quencher group (for use with ABI series real-time fluorescence quantitative PCR instruments, Falls benötigt, Wenden Sie sich im Voraus an den Hersteller oder fügen Sie den ROX-Kalibrierfarbstoff selbst hinzu, and select ROX as the quencher group; ansonsten, Folgen Sie dem normalen Verfahren).

• Ergebnisermittlung

4.1 Ct value of the positive control < 30 und ein „S“ shaped amplification curve appears, the test result is valid. If not, Der Versuch sollte wiederholt werden. If the retest is still invalid, Bitte wenden Sie sich an das technische Personal.

4.2 Sample detection result: Ct-Wert ≤ 38 und ein „S“ shaped amplification curve appears, indicating a positive result, which means the sample contains White Spot Syndrome Virus (WSSV).

4.3 Sample detection result: 38 < Ct < 40, es gilt als verdächtig. The sample should be retested. If the retest Ct value < 40 and there is a clear amplification curve, Es wird als positiv angesehen; ansonsten, Es wird als negativ angesehen.

4.4 Sample detection result: Kein Ct-Wert oder Ct-Wert ≥ 40 und nein „S“ geformte Verstärkungskurve, Es wird als negativ angesehen, indicating that White Spot Syndrome Virus (WSSV) was not detected in the sample.

Vorsichtsmaßnahmen：

• This test kit has high sensitivity. Um eine Kontamination zu verhindern, the experiment needs to be strictly partitioned, and physical isolation between partitions is recommended to avoid cross-contamination caused by human factors.
• Tragen Sie während des Experiments Laborkittel und Latexhandschuhe. Use separate tools in different areas, and remember to change gloves and lab coats.
• The components in the reaction solution are light-sensitive and should be stored away from light. Tauen Sie die Reagenzien vor der Verwendung vollständig auf, aber vermeiden Sie wiederholtes Einfrieren und Auftauen.
• Follow the instructions strictly for steps such as reagent preparation and sample addition. Disinfect the workbench, Zentrifuge, and pipettor regularly using chlorine disinfectants, Ethanol, Nukleinsäure-Dekontaminationsmittel, or ultraviolet light.
• Nach der Reaktion, place the PCR reaction tubes in sealed bags for disposal. Do not open the tubes to avoid aerosol contamination.
• Do not mix reagents from different batches. Use within the expiration date. Consumables should be treated without enzymes and sterile.
• A negative test result does not completely indicate a non-infected state; it is closely related to the quality of the obtained nucleic acid. „Negativ“ results may be due to unqualified nucleic acid sample quality, low sample nucleic acid load, or unsuccessful nucleic acid extraction (Erkennung). Veterinary clinical considerations are necessary for specific diagnoses.
• Possible causes of false positives: Cross-contamination during sample collection, Transport, and nucleic acid extraction.
• For any other questions, please contact the manufacturer’s technical staff promptly. Dieses Produkt dient nur zur Verwendung von wissenschaftlicher Forschung und ist nicht für klinische Diagnose oder andere Zwecke bestimmt.