Einführung
- The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
- Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 Zu 11.0 during nucleic acid extraction.
- Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 Zu 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
- Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. Typically, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
- This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikation | |
| Grundlegende Inhalte | Empfohlene Anwendung | Nukleinsäureextraktion |
| CAS-Nummern | 1185-53-1 | |
| Formel | C4H11NO3.HCL | |
| Molekulargewicht | 157.60 | |
| Stabilität | Unbegrenzt unter trockenen Bedingungen | |
| Ebene | Molekularbiologie | |
| Aussehen | Weißer Kristall | |
| Reinheit | ≥99 % | |
| Transportbedingungen | Zimmertemperatur | |
| Aufbewahrungsbedingungen | Zimmertemperatur | |
| Verunreinigungsparameter | Geruch | Nichts |
| Melting point ℃ | 145-155 | |
| Heavymetal | <5 PPM | |
| Losson drying | ≤0.09% | |
| UV-Absorptionswert | Absorptionswert @ 230 (5%) | ≤0,01 |
| Absorptionswert @ 260 (5%) | ≤0,01 | |
| Absorptionswert @ 280 (5%) | ≤0,01 | |
| Absorptionswert @ 320 (5%) | ≤0,01 | |
| Im Zusammenhang mit der Extraktion von Nukleinsäuren | DNase-Test (0.5M) | Nicht erkannt |
| RNase-Test (0.5M) | Nicht erkannt | |
| PH Wert (2M) | 4.0-5.0 |
Bestellinformationen
| Katze nein. | Produktname | Paket |
| C11224 | Tris-hcl, TRIS hydrochloride
(Molekularbiologie) |
1KG |
| C11225 | 5KG |






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