- Produktbeschreibung:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Beispieltypen:
- Vollblut
- Buffy-Mantel
- Knochenmark
- Body fluids
- Purification Time: As little as 25 Protokoll.
- Merkmale:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from whole blood using the economic salt out method |
| Anwendungen | PCR, Enzymverdauung, Southern hybridization, usw. |
| Reinigungstechnologie | Salting out method |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Animal tissue |
| Probenmenge | Unlimited |
| Elutionsvolumen | ≥100μl |
| Zeit pro Lauf | Variation with sample amount |
Principles
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- Verunreinigungsentfernung:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 Und 1.9.
- DNA size can reach up to 200 kb.
Vorteile
- Sicher – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Hohe Reinheit – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Inhalt des Kits
| Inhalt | D331101 | D331102 | D331103 |
| Purification Volumes | 50 ml | 300 ml | 1000 ml |
| 10 x Buffer RBC | 20 ml | 100 ml | 3 X 100 ml |
| Buffer WTL | 60 ml | 350 ml | 1000 ml |
| Buffer PPS | 20 ml | 120 ml | 350 ml |
| RNase-Lösung | 300 µl | 1.8 ml | 6 ml |
| Buffer TE | 10 ml | 30 ml | 120 ml |
Lagerung und Stabilität
- RNase Solution Storage:
- Bei der Ankunft, Lagern Sie bei 2-8 ° C..
- Kurzfristige Lagerung (bis zu 24 Wochen) bei Raumtemperatur (15-25°C) does not affect performance.
- Verbleibende Kit -Komponenten:
- Dry components can be stored at room temperature (15-25°C).
- Stabil für zumindest 18 Monate unter diesen Bedingungen.
- Entire Kit Storage:
- Das gesamte Kit kann bei 2–8 °C gelagert werden.
- Puffer sollten vor dem Gebrauch wieder aufgelöst werden, wenn sie bei dieser Temperatur gespeichert werden.
- Stellen Sie sicher, dass alle Puffer bei der Verwendung bei Raumtemperatur stehen.
