Spezifikation
| Attribut | Einzelheiten |
|---|---|
| Lagerung | 2-8℃ |
| Einheit | Box |
| Gültigkeitsdauer | 6 Monate |
| Englischer Name | Pepsase Activity Assay Kit |
| Erkennungsmethode | Spektrophotometer/Mikroplattenleser |
| Spezifikation | 100T/48S |
Produktzusammensetzung
| Reagenzname | Spezifikation | Storage Condition |
|---|---|---|
| Extraktionslösung | Flüssig 50 mL×1 bottle | Speichern Sie bei 4 ℃ |
| Reagenz eins | Powder×1 bottle | Speichern Sie bei 4 ℃ |
| Reagenz zwei | Flüssig 15 mL×1 bottle | Speichern Sie bei 4 ℃ |
| Reagenz drei | Powder×1 bottle | Speichern Sie bei 4 ℃ |
Instructions for preparing the solutions:
- Solution One: Hinzufügen 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: Hinzufügen 10 mL of distilled water before use and dissolve completely.
Produktbeschreibung:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Eis
- Destilliertes Wasser
Notiz: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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