Einführung
- Extracts DNA from various sources: Gewebe, Zellen, Blut, Speichel, Tupfer, Blutflecken, Samen, und andere klinische Proben.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, Southern Blot, viral DNA testing, und mehr.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total DNA from 1-100ul blood, FFPE, Gewebe, und andere Proben |
| Anwendungen | Sequenzierung der zweiten Generation, PCR, Echtzeit-PCR, usw. |
| Reinigungsmethode | Monodisperse magnetic beads |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Manuell oder automatisch |
| Beispielstyp | Antikoagulierendes Blut, konzentriertes Blut, Buffy-Mantel, Lymphozyten, und kultivierte Zellen |
| Probenmenge | Body fluid : 10 – 200μl, Gewebe : ≤10mg |
| Ertrag | 10ng – 15μg |
| Elutionsvolumen | 80 -100μl |
| Zeit pro Lauf |
Prinzip
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- Die Probe erfährt Lyse und Verdauung durch Lysat und Protease, releasing DNA into the solution.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, Buffy-Mantel, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
Inhalt des Kits
| Inhalt | IVD3101 |
| Reinigungszeiten | 200 |
| Partikel binden | 4.5 ml |
| Proteinase K | 90 mg |
| Protease-Auflösungspuffer | 10 ml |
| Puffer-ATL | 60 ml |
| Puffer AL | 60 ml |
| Puffer BD* | 20 ml |
| Puffer BXW1* | 110 ml |
| Elutionspuffer | 30 ml |
Lagerung und Stabilität
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- Kurzfristige Lagerung (bis zu 18 Wochen) bei Raumtemperatur (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) und bleiben zumindest stabil 18 Monate unter diesen Bedingungen.
