MagPure A4 XP Features:
- Technology Base:
- Employs Magen’s solid-phase paramagnetic bead technology designed for the purification of PCR amplicons.
- High-Throughput Capability:
- Suitable for high-throughput settings, allowing for efficient simultaneous processing of multiple samples.
AmPure Technology:
- Selektive Bindung:
- Uses an optimized buffer that selectively binds PCR amplicons 100bp and larger to paramagnetic beads.
- Verunreinigungsentfernung:
- Simple washing procedure efficiently removes excess primers, Nukleotide, salts, and enzymes.
- Purity of PCR Product:
- Results in PCR products that are virtually free from contaminants, suitable for precise downstream applications.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
| Anwendungen | NGS, DNA library |
| Reinigungstechnologie | Magnetperlen-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | DNA-Produkte, restriction endonuclease systems, or other enzymatic reaction solutions |
| Probenmenge | Appropriate |
| Erholung | 80% |
| Operationszeit | ≤ 50 Minuten |
Prinzip
Reinigungsmethode:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Verunreinigungsentfernung:
- Excess primers, Nukleotide, salts, and enzymes are removed through a simple washing procedure.
- DNA -Elution:
- Purified DNA is eluted using Elution Buffer or Water.
Vorteile
- High recovery — up to 80%
- High throughput — using magnetic beads purification technology
Inhalt des Kits
| Inhalt | BP-5 | BP-50 | BP-500 |
| MagPure A4 XP | 5 ml | 50 ml | 500 ml |
Lagerung und Stabilität
Storage and Handling Instructions:
- Lagerbedingungen:
- Store MagPure A4 XP at 2-8°C upon arrival.
- Stable for up to 18 Monate unter diesen Bedingungen.
- Short-term Storage:
- Kurzfristige Lagerung (bis zu 4 Wochen) bei Raumtemperatur (15-25°C) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogeneous and consistent in color.
DO NOT FREEZE.
Rezensionen
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