The Kit integrates phenol/Guanidin-based lysis and silica membrane purification of total RNA. Magzol -Reagenz, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples,Bakterien, cells and other samples.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total RNA (include miRNA) from 100mg lipid tissue, Gewebe, Zelle, plant, body fluids using columns and MagZol reagent |
| Anwendungen | RT-PCR, qRT-PCR, Nordhybridisierung, second generation sequencing |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Animal tissue, muscle fiber containing tissue,adipose tissue, kultivierte Zellen, Lymphozyten, simple plants and other biological samples |
| Probenmenge | Animal tissue sample: 1-60mg (spleen≤ 10mg)
Kultivierte Zellen: 5 X 106 Plant leaves: 50-100mg |
| Ertrag | 2-200μg |
| Elutionsvolumen | ≥50μl |
| Zeit pro Lauf | ≤30 Minuten (1–24 Proben) |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100µg |
Prinzip
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Vorteile
- Efficient DNA removal – one step RNA-Extraktion can effectively remove genomic DNA
- Gute Qualität – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Schnell – Es können mehrere Proben entnommen werden 30 Protokoll
- High applicability – samples including animals, Pflanzen, Bakterien, Zellen, usw.
- High output – enable to process large samples and the yield can be up to 200μg
Inhalt des Kits
| Inhalt | R413002 | D413003 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| 2ml-Sammelröhrchen | 50 | 250 |
| Magzol -Reagenz | 60 ml | 270 ml |
| Puffer RW1 | 50 ml | 200 ml |
| Puffer RW2* | 20 ml | 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
MagZol Reagent should be stored at 2-8°C upon arrival. Jedoch, kurzfristige Lagerung (bis zu 12 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf seine Leistung. Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.
Experimentdaten
Rezensionen
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