Einführung
The HiPure Liquid RNA & miRNA Kit integrates phenol/Guanidin-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini Spin-Säule.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Anwendungen | qPCR / RT-PCR, liquid or solid-phase chip analysis, Hybridisierung, und SNP -Erkennung |
| Reinigungsmethode | Mini-Spin-Säule |
| Reinigungstechnologie | Silica-Technologie, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Blut, Serum, Plasma |
| Probenmenge | 250μl |
| Elutionsvolumen | ≥20μl |
| Flüssigkeitstransportvolumen pro Säule | 800µl |
| Bindungsausbeute der Säule | 100μg |
Prinzip
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (bis zu 100 µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Vorteile
- Gute Qualität – high purity total RNA can be directly used in various sensitive downstream applications
- Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
- Safety – no phenol-chloroform extraction is required
- Sensitive – direct lysis of blood, Plasma, and other samples without separation of leukocytes
Inhalt des Kits
| Inhalt | R416302 | R416303 |
| Reinigungszeiten | 50 Vorbereitungen | 250 Vorbereitungen |
| HiPure RNA Mini-Säulen | 50 | 250 |
| 2ml-Sammelröhrchen | 50 | 2 X 125 |
| MagZol LS Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Puffer RW2* | 20 ml | 2 X 50 ml |
| RNase-freies Wasser | 10 ml | 30 ml |
Lagerung und Stabilität
MagZol LS Reagent should be stored at 2-8°C upon arrival. Jedoch, kurzfristige Lagerung (bis zu 24 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können trocken bei Raumtemperatur gelagert werden (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen.
Rezensionen
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