This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 分分鐘. Purified RNA is ready for downstream applications such as RT-聚合酶鍊式反應, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total RNA from tissue and cells and plant |
| 應用領域 | 逆轉錄聚合酶連鎖反應, cDNA合成, 二代定序 |
| 純化方法 | 多分散磁珠 |
| 淨化技術 | 磁珠技術 |
| 工藝方法 | 手動或自動 |
| 自適應儀器 | 核酸萃取儀, 移液工作站 |
| 樣品類型 | 動物組織, human tissue, 培養細胞, 淋巴球, ordinary plant tissue |
| 樣品量 | 細胞: 1 X 107
動物組織: ≤20mg 植物組織: ≤100mg Yeast cell: ≤5 x 106 |
| 屈服 | 5-100微克 |
原則
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
優點
- 高純度 – 的 260 / 280 :1.9-2.0, 的 260 / 230 :1.5-2.0
- 經濟 – 少於 50% Qiagen等進口產品的價格
- 自動的 – 無需手動參與的提取, 節省時間和精力
- 普遍的 – suitable for various animal tissues, cultured cells and conventional plant fungal tissues
套件內容
| 內容 | R662201 | R662202 | R662203 |
| 淨化時間 | 48 準備工作 | 96 準備工作 | 5 X 96 準備工作 |
| MAGPURE RNA顆粒 | 1.7 毫升 | 4.0 毫升 | 18 毫升 |
| 蛋白酶K | 24 毫克 | 50 毫克 | 240 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 5 毫升 | 15 毫升 |
| 脫氧核糖核酸酶I | 600 微升 | 2 X 600 微升 | 10 X 600 微升 |
| 脫氧核糖核酸酶緩衝液 | 30 毫升 | 40 毫升 | 200 毫升 |
| RTL 裂解緩衝液 | 40 毫升 | 80 毫升 | 400 毫升 |
| 緩衝區MCB* | 18 毫升 | 30 毫升 | 150 毫升 |
| 緩衝器 MW1* | 44 毫升 | 66 毫升 | 2 X 220 毫升 |
| 緩衝器 RW2* | 20 毫升 | 50 毫升 | 2 X 100 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 | 120 毫升 |
儲存和穩定性
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. 然而, 短期儲存 (DNase I 高達 1 週, MagPure RNA Particles and Proteinase K up to 8 週) 在室溫下 (15–25°C) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15–25°C) and are stable for atleast 18 在這些條件下幾個月.
評論
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