模糊顆粒g

介紹

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, 或矽組. 基於矽的磁珠是最常見的, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, 凝膠DNA恢復, 產品純化, 基因組DNA和 RNA萃取, and viral nucleic acid extraction.

細節

規格

原則

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, 模糊顆粒 can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

酒精介導的結合: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (核醣體和核小體) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, 將DNA/RNA磁珠複合物重懸於滅菌水或TE緩衝液中, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

訂購資訊

採購指南

• The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA在混合鹽的存在下與磁顆粒的二氧化矽表面結合. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, 核苷酸, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.