產品概述
| 特徵 | 規格 |
| 專注 | 100 毫克/毫升 |
| 外貌 | 黑色顆粒的懸架 |
| 表面功能組 | si-oh, 矽醇 |
| 不同 | 多分散的無定形 |
| 粒度 | 1.5-5 微米 |
| 保存條件 | 室內溫度, 有效 2 年.
建議存儲在2-8°C下以防止微生物生長. |
| 磁反應速度 | 15-30 秒 |
| 定居速度 | >5 分分鐘 |
| 高鹽介導的結合 | >2中號 胍 異硫氰酸鹽, DNA恢復到 80% |
| 酒精介導的結合 | 2M鹽烷鹽酸/異丙醇 (30%), 和DNA的恢復 / RNA高如 85% |
| PEG8000介導的結合 | DNA/RNA的恢復已達到 85% |
| DNase/RNase | 沒有檢測到 |
| DNA殘基 | <1 ppm |
| 推薦應用 | 質粒萃取, 凝膠DNA恢復, 基因組DNA提取 和 RNA萃取. |
原則
Magnetic Separation:
- Magnetic beads with silica coating are used.
- After lysing the sample, DNA/RNA is released.
- Magnetic beads and binding solution are added.
- DNA/RNA binds to the surface of the beads.
- A magnet separates the bead-DNA/RNA complex from the solution (impurities).
選擇性結合:
- 高鹽介導的結合 (2-4M guanidine isothiocyanate): selectively recovers DNA molecules.
- 酒精介導的結合 (guanidine salt and >25% 酒精): selectively recovers DNA/RNA molecules.
- Proteins and other impurities are not adsorbed to the beads.
洗脫:
- The purified DNA/RNA complex is washed to remove contaminants.
- DNA/RNA is released from the beads using sterilized water or TE buffer.
評論
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