介紹

• 目的: Extraction of stool RNA.
• 樣品相容性: Suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples.
• 科技: Utilizes silica gel column purification technology and an original solution system.
• Key Feature: Effectively removes humic acid and other inhibitory factors present in stool samples.
• 應用領域: The purified RNA can be directly used in RT-聚合酶鍊式反應, 北方雜交, and other experiments.

規格

原則

• Principle of HiPure Silica Gel Column: Utilizes a high binding ability glass fiber filter membrane as the substrate. Under high concentrations of ionizing agents (例如, Guanidinium chloride or 胍 異硫氰酸鹽), nucleic acids are adsorbed through hydrogen bonding and electrostatic interactions, while proteins and other impurities are not adsorbed and removed. 隨後, the filter membrane with adsorbed nucleic acids is washed to remove proteins and salts. 最後, low salt buffer solution (例如, 緩衝區TE) or water is used to elute the purified nucleic acids, resulting in high purity suitable for downstream experiments.
• 樣品加工: Stool samples are homogenized in the lysis solution, then further lysed in a high-temperature water bath to release RNA into the solution. Chloroform extraction is employed to remove genomic DNA and impurities. The supernatant is transferred to an alcohol-free binding solution, followed by RNA purification through a column. 最後, RNA is eluted using RNase Free Water.
• 應用領域: The purified RNA is suitable for various downstream experiments such as PCR, Southern hybridization, and enzyme digestion.

優點

• High Purity: Utilizes a unique adsorbent for more efficient removal of inhibitors, ensuring high purity of extracted RNA.
• High Concentration: Achieves maximum extraction of total RNA from stool samples due to its high concentration, maximizing RNA yield.
• 敏感的: Capable of purifying RNA at the level of picograms (pg), ensuring sensitivity in downstream applications.

套件內容

儲存和穩定性

• 儲存條件: Kit components can be stored at room temperature (15–25°C) and remain stable for 18 在這些條件下幾個月.
• Buffer SPL: May form precipitates at low temperatures. Dissolve precipitates by placing the Buffer SPL in a 55°C water bath.
• 緩衝phc: After receiving the product, store Buffer PHC at 2-8°C.