HiPure 質粒迷你試劑盒

介紹

• 快速地, 簡單的, and Cost-Effective: Offers a rapid, easy-to-use, and economical method for 質粒DNA miniprep suitable for routine molecular biology applications.
• Silica Membrane Technology: Utilizes silica membrane technology, eliminating the need for cumbersome steps associated with loose resins or slurries, streamlining the purification process.
• Immediate Usability: Purified plasmid DNA is immediately ready for downstream applications, 節省時間和精力.
• No Phenol Extraction or Ethanol Precipitation: Bypasses the need for phenol extraction and ethanol precipitation, simplifying the workflow and reducing hands-on time.
• 高質量的DNA: Yields high-quality plasmid DNA eluted in a small volume of Tris buffer or water, ensuring optimal performance in downstream applications.

規格

原則

• Alkaline Lysis: Begins with the alkaline lysis of bacterial cells, which breaks down the cell membrane and releases plasmid DNA into the lysate.
• Silica-Based DNA Binding: The released DNA is adsorbed onto the silica membrane in the presence of high salt, facilitating efficient binding of DNA to the membrane surface.
• Three Basic Steps: The procedure involves three main steps: (1) Preparation and clearing of the bacterial lysate using an alkaline method, (2) Transfer of the cleared lysate to a column for DNA binding, 和 (3) Washing to remove proteins and other impurities.
• Silica Membrane: Utilizes a unique silica membrane in the kit, which completely replaces traditional glass or silica slurries typically used in plasmid DNA minipreps.
• Elution with Low-Salt Buffer: After washing to remove impurities, 核酸, including plasmid DNA, is eluted from the silica membrane using a low-salt buffer containing 10mM Tris, 酸鹼度 9.0, and 0.5mM EDTA.

優點

• 高純度 – 純化的質粒可以直接用於測序, enzyme digestion PCR, ETC.
• 快速地 – 它只需要 1 minute to obtain supernatant by optimized solution (10 其他品牌的分鐘)
• 高產 – up to 35µg plasmid can be bound in one column

套件內容

儲存和穩定性

• 儲存條件: Kit components can be stored dry at room temperature (15-25℃) and remain stable for a minimum of 18 在這些條件下幾個月.
• Buffer Precipitates: In case of any precipitates forming in the buffers, warm them at 37°C to dissolve effectively.
• Buffer P1 Stability: After the addition of RNase A, Buffer P1 remains stable for up to 6 存儲在2-8°C的月份.

實驗數據

採購指南