Hipure質粒EF迷你套件

介紹

HiPure Plasmid EF Mini Kit Overview:

1. Technology Integration:
   • Combines HiPure technology with Magen’s innovative Endotoxin Removal Technology (ETR) to ensure high-quality plasmid DNA純化.
2. Endotoxin Management:
   • Features a specially formulated buffer designed to prevent endotoxins from binding to the HiPure matrix, significantly reducing endotoxin levels in the final DNA product.
3. Application Suitability:
   • Provides plasmid DNA ideal for eukaryotic transfection and in vitro experiments, especially where low endotoxin levels are critical.
4. Impact of Endotoxin Contamination:
   • Endotoxin contamination can drastically lower transfection efficiencies, particularly in endotoxin-sensitive cell lines.
5. Clinical Relevance:
   • In contexts such as gene therapy, controlling endotoxin levels is crucial due to the potential for serious adverse effects like fever, endotoxin shock syndrome, and negative impacts on in vitro transfection into immune cells.

細節

規格

原則

Hipure質粒程序基於細菌細胞的鹼性裂解，然後在高鹽存在下將DNA吸附到二氧化矽上. 套件中使用的獨特二氧化矽膜完全取代了質粒DNA小型玻璃或二氧化矽漿液. 該過程包括 3 基本步驟: 通過鹼方法製備和清除細菌裂解物，然後將上清液轉移到柱中以結合DNA. 洗滌蛋白質及其他雜質後, 核酸最終用低鹽緩衝液洗脫 (10毫米三羥甲基氨基甲烷, 博士學位, 0.5乙二胺四乙酸).

優點

• 高純度 – 純化的質粒可以直接用於測序, 酶消化和 聚合酶鍊式反應, ETC.
• 快速地 – 二氧化矽凝膠柱純化比離子交換快得多
• 高產 – up to 70μg plasmid can be binded in one column
• 低內毒素含量 – 獲得的質粒可直接用於細胞轉染和動物注射

套件內容

儲存和穩定性

儲存說明:

1. General Storage:
   • Kit components can be stored dry at room temperature (15–25°C).
   • 至少穩定 18 在這些條件下幾個月.
2. Handling Precipitates:
   • 如果緩衝液中形成任何沉澱物, warm at 37°C to dissolve.
3. Specific Storage for Buffer P1 with RNase A:
   • 添加RNase A後, 緩衝液P1穩定 6 存儲在2–8°C的月份.

採購指南