This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 分分鐘. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-聚合酶鍊式反應, 印跡雜交, poly A+ purification, nucleic acid protection, 和體外翻譯.
細節
套件內容
| 內容 | R415002 | D415003 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure RNA 迷你柱 | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2毫升收集管 | 100 | 500 |
| TCEP (1中號) | 0.29 G | 5 X 0.29 G |
| Buffer EP | 1.0 毫升 | 5.0 毫升 |
| Buffer PSL | 50 毫升 | 250 毫升 |
| 緩衝器RW1 | 50 毫升 | 250 毫升 |
| 緩衝器 RW2* | 20 毫升 | 2 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
儲存和穩定性
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) 並且至少穩定 18 在這些條件下幾個月. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
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