介紹
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. 純化的 DNA/RNA 用於 RT-聚合酶鍊式反應 和PCR檢測.
細節
規格
| 特徵 | 規格 |
| Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
| 應用領域 | 逆轉錄聚合酶連鎖反應, cDNA合成, PCR and second-generation sequencing, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | FFPE slice, FFPE embedded tissue |
| 樣品量 | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
原則
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. 離心後, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, 而蛋白質不被吸附並透過過濾去除. 洗滌蛋白質及其他雜質後, RNA was finally eluted with low-salt buffer. 為了 DNA純化, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, 而蛋白質不被吸附並透過過濾去除. 洗滌蛋白質及其他雜質後, DNA was finally eluted with low-salt buffer.
優點
- 高品質 – 高純度總RNA / DNA can be directly used in various downstream applications
- 快速地 – 列方法可以完成在中提取幾個樣品 30 分分鐘
- 安全的 – 無需苯酚氯仿萃取
- 同時提取 – 同時將DNA和RNA與一個樣品分離
套件內容
| 內容 | IVD5116 |
| 淨化時間 | 50 準備工作 |
| HiPure DNA Micro Column | 50 |
| HiPure RNA Mini Column I | 50 |
| 2毫升收集管 | 150 |
| 蛋白酶K | 50 毫克 |
| 蛋白酶溶解緩衝液 | 5 毫升 |
| 緩衝DPS | 60 毫升 |
| Buffer FRL | 15 毫升 |
| 緩衝區ATL | 15 毫升 |
| 緩衝RLC | 15 毫升 |
| 緩衝液AL | 15 毫升 |
| Buffer VHB | 44 毫升 |
| 緩衝區RW2 | 25 毫升 |
| 無核糖核酸酶水 | 10 毫升 |
| 緩衝AE | 10 毫升 |
儲存和穩定性
蛋白酶K到達後應在2-8°C下儲存. 然而, 短期儲存 (取決於 12 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘試劑盒成分可在室溫下保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月.
實驗數據
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