HiPure FFPE DNA 試劑盒

The HiPure FFPE Nucleic Acid Kit offers a fast and user-friendly solution for extracting DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.

• Target Samples: FFPE tissue and sections
• Simple and Rapid: DNA extraction can be completed in just 20 分分鐘 (excluding digestion time).
• Silica Gel Technology: Employs a reliable silica gel column purification method, eliminating the need for harsh organic solvents (phenol-chloroform) or alcohol precipitation steps.
• 下游應用程序: Purified DNA is suitable for various analyses like 聚合酶鍊式反應 (聚合酶鍊式反應), 南方印跡, 和病毒DNA檢測.

好處:

• 簡化的工作流程: The silica gel column technology offers a faster and less hazardous alternative to traditional methods.
• 高質量的DNA: The extraction process ensures the isolation of DNA suitable for diverse downstream applications.
• 廣泛的適用性: The kit works effectively with various FFPE tissue samples.

This kit empowers researchers working in various fields, 包括腫瘤學, pathology, 和傳染病, to efficiently extract DNA from archived FFPE samples for further analysis.

規格

特徵	規格
主要功能	Isolation of total DNA from FFPE tissue samples
應用領域	聚合酶鍊式反應, southern blot viral DNA detection, ETC.
純化方法	小型的 旋轉柱
淨化技術	二氧化矽技術
工藝方法	手動的 (離心或真空)
樣品類型	Formalin-fixed, paraffin-embedded (FFPE) tissue and section samples
樣品量	<20毫克
洗脫體積	<20微升
每次運行時間	<跨距 “=”” 風格=”大綱: 0像素; 盒子尺寸: 邊框; 字體大小: 16像素;”>≤20 minutes
每根柱的載液量	4毫升
柱結合率	100微克

原則

HiPure FFPE Nucleic Acid Kit, designed for rapid and efficient extraction of both DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples:

主要特點:

• Silica Gel Column Technology: Utilizes a reliable and streamlined approach, eliminating the need for harsh chemicals like phenol-chloroform.
• Fast Processing: Extracts DNA/RNA in just 20 分分鐘, excluding digestion time.
• 樣品相容性: Works effectively with various FFPE tissue sections.

提取過程:

1. Deparaffinization: Xylene removes paraffin wax, a common component used to preserve tissues.
2. 裂解和消化: A special solution (lysate) and an enzyme (protease) break down cells and proteins, releasing nucleic acids (DNA and RNA) into the solution.
3. De-Crosslinking: Heating at 90°C reverses the effects of formalin fixation, allowing easier access to nucleic acids.
4. 與二氧化矽膜結合: After adjusting conditions with ethanol, the sample is transferred to a column. DNA/RNA binds to the silica membrane, while proteins pass through.
5. 洗滌: Buffers remove impurities like proteins and salts from the captured nucleic acids.
6. 洗脫: A low-salt buffer elutes the purified DNA/RNA, making it ready for downstream applications.

應用領域:

• 聚合酶鍊式反應 (聚合酶鍊式反應)
• 南方印跡
• Viral nucleic acid detection
• Other analyses requiring high-quality DNA or RNA

優點

The HiPure FFPE Nucleic Acid Kit offers a superior solution for extracting DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.

安全:

• No Xylene Contact: Eliminates the need for hazardous xylene, minimizing exposure to harmful chemicals.

速度:

• Fast Processing: Extracts DNA/RNA in just 20 分分鐘 (excluding digestion time), allowing you to process multiple samples within 2 小時.
• No Overnight Incubation: Streamlines the workflow by eliminating the need for lengthy overnight incubation steps.

高效率:

• Enhanced PCR Sensitivity: Effectively removes formaldehyde modifications from DNA, significantly improving the sensitivity of downstream PCR (聚合酶鍊式反應) 分析.

套件內容

內容	D312602	D312603
淨化時間	50 準備工作	250 準備工作
HiPure DNA 迷你柱 I	50	250
2毫升收集管	50	250
緩衝DPS	30 毫升	150 毫升
緩衝區ATL	15 毫升	60 毫升
緩衝液AL	15 毫升	60 毫升
緩衝區GW1*	22 毫升	88 毫升
緩衝器 GW2*	12 毫升	50 毫升
蛋白酶K	24 毫克	120 毫克
蛋白酶溶解緩衝液	1.8 毫升	10 毫升
緩衝AE	10 毫升	30 毫升

儲存和穩定性

• 蛋白酶K: Keep it cool in the refrigerator (2-8℃) 抵達後發揮最大功效.
• Short-Term Storage (取決於 12 週): Proteinase K can last at room temperature (15-25℃) 最多為 12 weeks without issues.
• Everything Else: Store other kit components at room temperature (15-25℃). 他們至少穩定 18 在這些條件下幾個月.
• Important: 如果存儲整個套件冷, remember to warm up the buffers before use. All buffers should be at room temperature for optimal results.

實驗數據