HiPure 循環核酸微量試劑盒, is designed to efficiently purify short nucleic acids (<1000BP) from human biological fluids.
- Target Molecules:
- Tumor-derived cell-free DNA fragments (遊離DNA) and mRNA in blood
- 母血中的胎兒核酸
- Free-circulating cell-free RNA (cfRNA)
- Viral nucleic acids
- 樣品相容性:
- 人類血漿
- 人血清
- 人類尿
- 樣品可以是新鮮的或冷凍的 (僅一次)
- 高效淨化: The kit isolates short nucleic acids present in biofluids.
- High Purity: Purified nucleic acids are free of proteins, nucleases, 和其他污染物.
- 下游應用程序: The eluted nucleic acids are ready for use in various downstream analyses, 包括:
- Amplification reactions (例如, 聚合酶鍊式反應)
- Long-term storage (-30°C to -15°C)
好處:
- Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
- 廣泛的樣品相容性: 與各種人體生物體液有效配合.
- 高質量的結果: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
- Convenient Storage: Purified nucleic acids can be stored for long-term use.
This kit empowers researchers studying circulating nucleic acids in various fields, 包括腫瘤學, 非侵入性產前檢測 (NIPT), and viral diagnostics.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation circulating DNA from 0.6ml plasma, 血清, 體液 |
| 應用領域 | 定量PCR, 液體或固體晶片分析, 雜交, 和SNP檢測, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 血清, 電漿, and other cell-free fluid samples |
| 樣品量 | 0.6毫升 |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤40分鐘 |
| 每根柱的載液量 | 800微升 |
| 柱結合率 | 100微克 |
原則
This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:
1. 裂解和消化:
- The sample is treated with a lysate solution and protease to break open cells and degrade proteins. This releases DNA into the solution.
2. 與二氧化矽膜結合:
- 將含有 DNA 的裂解液轉移至吸附板或過濾管柱. 由於有利的相互作用，這些工具中的二氧化矽膜選擇性地結合 DNA 分子, 而蛋白質和其他雜質則透過.
3. 洗滌:
- 洗滌緩衝液通過色譜管柱, 去除未黏附在二氧化矽上的未結合蛋白質和細胞碎片.
4. 洗脫:
- A low-salt buffer is used to elute the purified DNA from the silica membrane. 這會破壞 DNA 和二氧化矽之間的相互作用, allowing collection of purified DNA in a separate tube.
此方法為需要純化 DNA 進行分析的各個領域的研究人員提供了寶貴的工具, 例如PCR, 南方印跡, 和下一代定序.
優點
- 高效淨化: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
- 高質量的DNA: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
- 簡化的工作流程: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
套件內容
| 內容 | D318002 | D318003 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| 緩衝區ACL | 40 毫升 | 200 毫升 |
| Buffer DCW1 | 22 毫升 | 110 毫升 |
| 緩衝器 DCW2* | 20 毫升 | 2 X 50 毫升 |
| 蛋白酶K | 34 毫克 | 180 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 | 10 毫升 |
| 載體RNA | 110 微克 | 310 微克 |
| 無核酸酶水 | 10 毫升 | 30 毫升 |
| HiPure CFDNA 迷你柱 | 50 | 250 |
| 2 毫升收集管 | 100 | 5 X 100 |
儲存和穩定性
Optimal Performance:
- Store Proteinase K and Carrier RNA at 2-8°C (冰箱) upon arrival for best results.
可接受的短期儲存:
- Proteinase K and Carrier RNA can be stored for up to 12 室溫下的幾週 (15-25℃) 不影響他們的表現.
其餘套件組件:
- Store all other components of the kit dry at room temperature (15-25℃).
- 他們至少穩定 18 在這些條件下幾個月.
重要提示:
- 如果將整個套件儲存在 2-8°C, 使用前確保重新溶解緩衝區.
- Always warm all buffers to room temperature before using them for optimal performance.
By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.
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